Enzymatic kinetic rate and end-point analyses of substrate, by use of a GeMSAEC fast analyzer.

Enzymatic substrate analysis is an attractive means of analysis in cliniaI chemistry because of its sensitivity and specificity. The GeMSAEC Fast Analyzer, in conjunction with a small computer, provides a means of performing routine enzymatic substrate analysis and offers the following advantages: (a) selectivity of approaches to enzymatic analysis, i.e., end-point or kinetic; (b) essentially parallel analyses of multiple samples, yielding a unique method for performing kinetic fixed-time analysis; (c) on-line data reduction, resulting in rapid calculation and output of results and the minimization of data handling errors; and (d) a small reagent volume per test (400 ILl), which reduces the cost of analysis. The analysis of substrate with enzymatic end-point and kinetic procedures is examined by use of a computerinterfaced Fast Analyzer. Computer programs were written to facilitate this study. Glucose (hexoki. nase/GPD), urea (urease/GMD), and uric acid (uricase) have been used as examples in eValuating both end-point and kinetic analyses. The advantages and limitations of each type of analysis are presented, with the emphasis being placed on enzymatic substrate analysis and means by which the computer-interfaced Fast Analyzer can facilitate both end-point and kinetic analyses.